Invitro Antimicrobial Efficacy of Jatropha Curcas Extracts on Escherichia Coli From Diabetic Females in Kitui County Kenya

Abstract

Background: Herbal usages for Urinary tract infections (UTI) in diabetes have been used for centuries in Kitui County. The plant Jatropha curcas is of numerous natural yields, traditional healers in Kitui County have been using various portions of the plant in different ways including bacterial infections treatment. Non-Communicable Diseases in Kenya gives 28% deaths where 2% was diabetes. Prevalence of diabetes in Kitui County is at 29. High glucose level in urine provides a conducive environment for pathogenic microorganisms Escherichia coli which is the utmost major cause of UTI at approximately 80% populating the colon.

Methods: This is an Experimental and Cross sectional method of research, in which the Escherichia coli clinical isolates were obtained from diabetic females identified with UTI at Kitui County Referrals Hospital and transported to Mount Kenya University. Crude Jatropha Curcas leaves and stem barks were harvested from Kitui County and Phytochemistry analysis done at East African Herbarium in the National Museums of Kenya, then after were subjected to clinical isolates of Escherichia coli for antimicrobial efficacy by evaluating their Minimum Inhibition Concentration and Minimum Bactericidal Concentration at Mount Kenya University, Microbiology Laboratory.

Results: Minimum Inhibition Concentrations produced Negative results and thus Minimum Bactericidal Concentration procedure was not done. A prevalence rate of 69% of Escherichia coli   isolates as the causative agent of UTIs in diabetic females of Kitui County, Kenya was noted.

Conclusion: Jatropha curcas is bacterial static against E. coli in Urinary Tract Infections of diabetic females located at Kitui County, Kenya.